Serotyping Streptococcus pneumoniae by Multiplex PCR
نویسندگان
چکیده
منابع مشابه
Serotyping Streptococcus pneumoniae by multiplex PCR.
The capsule is a major virulence factor of pneumococci, and it was shown that some capsular variants are associated with antimicrobial resistance and certain types of disease. Moreover, pneumococcal capsular typing has received renewed interest since the availability of conjugate vaccines, which include serotypes frequently associated with pediatric disease. Our aim was to develop a simple, rel...
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The introduction of pneumococcal conjugate vaccines necessitates continued monitoring of circulating strains to assess vaccine efficacy and replacement serotypes. Conventional serological methods are costly, labor-intensive, and prone to misidentification, while current DNA-based methods have limited serotype coverage requiring multiple PCR primers. In this study, a computer algorithm was devel...
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Background: Identifying regional types and evaluating the frequency of pneumococcal strains has become increasingly important especially in vaccination. The purpose of this study was the identification and frequency determination of our regional serotype and evaluation of the performance of recent type specific multiplex PCR for the diagnosis of streptococcus pneumonia serotypes. Methods: All ...
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Streptococcus pneumoniae is one of the major causes of pneumonia, meningitis and other pneumococcal infections in young children and elders. Determination of circulating S. pneumoniae serotypes is an essential service by public health laboratories for the monitoring of putative serotype replacement following the introduction of pneumococcal conjugate vaccines (PCVs) and of the efficacy of the i...
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The coagglutination test, which uses staphylococcal protein A, for serotyping strains of Streptococcus pneumoniae, was extended to include serotyping within serogroups. Serotyping was performed with "factor sera" prepared in the laboratory. Fifty one strains of S pneumoniae, which belonged to one of the seven serogroups included in the 14 valent vaccine formulation, were tested, and no inconsis...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2003
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.41.6.2378-2384.2003